The egs can be covalently linked to ml rna , the catalytic rna subunit of rnase p , forming a new kind of ribozyme , m1gs 将egs共价连接到m1rna的3末端成为附属于m1rna的一段引导序列,称为gs 。
Rnase p can be directed to cleave any rna when the target mrna is in a complex with a short , complementary oligonucleotide called an external guide sequence ( egs ) 能与mrna形成rnasep识别的底物形式的小片段rna称为外部引导序列egs ( externalguidesequence ) 。
Rnase p from e . coli is a ribonucleoprotein complex responsible for the 5 " maturation of trnas . the smallest substrate contains two important parts : ncca - 3 " terminal and rna helix area Rnasep是结构识别酶, m1rna底物至少包括两个要件: 1游离ncca - 3末端; 2特异互补的双链rna区域。